CoraLite® Plus 488-conjugated RCN1 Recombinant antibody

• 

  IF Staining of U2OS using CL488-83638-3

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 RCN1 antibody (CL488-83638-3, Clone: 240473E9 ) at dilution of 1:200, CL594-Phalloidin (red).

RCN1 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-83638-3

Clone No.240473E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

Reticulocalbin 1, 240473E9, PIG20, RCAL, RCN

---

Tested Applications

• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83638-3 targets RCN1 in applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RCN1 fusion protein Ag13256
• Full Name: reticulocalbin 1, EF-hand calcium binding domain
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 44-46 kDa
• GenBank Accession Number: BC010120
• Gene Symbol: RCN1
• Gene ID (NCBI): 5954
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15293
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Reticulocalbin-1 (RCN1) belongs to the CREC family. Reticulocalbin‐1, an endoplasmic reticulum protein, plays a crucial role in calcium homeostasis and inhibits ER stress‐induced apoptosis (PMID: 28319095). Its main functions include maintaining intracellular homeostasis and regulating cell proliferation and apoptosis, and it plays an important role in the development of various tumours (PMID: 38057406). RCN1 is expressed aberrantly and at a high level in various tumors, including acute myeloid leukemia (AML) (PMID: 37746742). In multiple cancers, such as glioblastoma, non‐small cell lung cancer, renal cell carcinoma, hepatocellular carcinoma, and oral squamous cell carcinoma, the overexpression of RCN1 has been observed indicating its involvement in tumorigenesis and invasion (PMID: 30172915, PMID: 34722631).