RCN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RCN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 10193-2-AP

Synonyms

ERC 55, E6BP, E6-binding protein, E6 binding protein, Calcium-binding protein ERC-55

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Validation Data Gallery

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  WB analysis using 10193-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10193-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10193-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 10193-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10193-2-AP

  IP result of anti-RCN2 (IP:10193-2-AP, 4ug; Detection:10193-2-AP 1:400) with mouse lung tissue lysate 2800ug.

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  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10193-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 10193-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse lung tissue, human brain tissue, HeLa cells
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human gliomas tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:350-1:1400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

10193-2-AP targets RCN2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RCN2 fusion protein Ag0247
• Full Name: reticulocalbin 2, EF-hand calcium binding domain
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC004892
• Gene Symbol: RCN2
• Gene ID (NCBI): 5955
• RRID: AB_2180018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.

Publications

Species	Application	Title
mouse	WB	Nat Med; Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial epilepsy.; Authors - Norihiko Yokoi
rat	WB	Cell Metab; A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.; Authors - Hui Peng
mouse	WB,IF	Proc Natl Acad Sci U S A; Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway and controls extraembryonic endoderm differentiation.; Authors - Li Lingjie L ; KD Validated
human	IF	Elife; Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking.; Authors - Pornchai Kaewsapsak
human	IF	Elife; Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation.; Authors - Victoria Hung
human	WB	Am J Cancer Res; YY1-mediated reticulocalbin-2 upregulation promotes the hepatocellular carcinoma progression via activating MYC signaling.; Authors - Chengjie Mei