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RCN2 Polyclonal antibody, PBS Only (10193-2-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 10193-2-AP (same clone as 10193-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
WB analysis of human brain using 10193-2-AP (same clone as 10193-2-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
WB analysis using 10193-2-AP (same clone as 10193-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 10193-2-AP (same clone as 10193-2-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IP experiment of mouse lung using 10193-2-AP (same clone as 10193-2-PBS)
IP result of anti-RCN2 (IP:10193-2-AP, 4ug; Detection:10193-2-AP 1:400) with mouse lung tissue lysate 2800ug. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IF Staining of HeLa using 10193-2-AP (same clone as 10193-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 10193-2-AP (same clone as 10193-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

RCN2 Polyclonal antibody, PBS Only

Name: RCN2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 10193-2-PBS

RCN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 10193-2-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Calcium binding protein ERC 55, Calcium-binding protein ERC-55, E6 binding protein, E6-binding protein, E6BP

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

10193-2-PBS targets RCN2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0247 Product name: Recombinant human RCN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-209 aa of BC004892 Sequence: KAEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLE Predict reactive species
Full Name	reticulocalbin 2, EF-hand calcium binding domain
Calculated Molecular Weight	37 kDa
Observed Molecular Weight	55 kDa
GenBank Accession Number	BC004892
Gene Symbol	RCN2
Gene ID (NCBI)	5955
RRID	AB_2180018
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14257
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.