RDBP Recombinant antibody, PBS Only

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  WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 85945-1-RR (same clone as 85945-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RDBP antibody (85945-1-RR, Clone: 250420G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

RDBP Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85945-1-PBS

Clone No.250420G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

NELFE, NELF E, NELF-E, RD, RNA-binding protein RD

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Product Information

85945-1-PBS targets RDBP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RDBP fusion protein Ag1186
• Full Name: RD RNA binding protein
• Calculated Molecular Weight: 37 kDa, 43 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC011600
• Gene Symbol: NELFE
• Gene ID (NCBI): 7936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P18615
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.