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  • Western Blot (WB) analysis of various lysates using RDBP Recombinant antibody (85945-1-RR)

    WB analysis using 85945-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RDBP Recombinant antibody (85945-1-RR)

    WB analysis using 85945-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RDBP Recombinant antibody (85945-1-RR)

    IF Staining of HeLa using 85945-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RDBP antibody (85945-1-RR, Clone: 250420G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RDBP Recombinant antibody

RDBP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85945-1-RR
Clone No.250420G2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

NELFE, NELF E, NELF-E, RD, RNA-binding protein RD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, Calu-3 cells, mouse brain tissue, rat brain tissue, HEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:7500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85945-1-RR targets RDBP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RDBP fusion protein Ag1186 Predict reactive species
Full Name RD RNA binding protein
Calculated Molecular Weight 37 kDa, 43 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC011600
Gene Symbol NELFE
Gene ID (NCBI) 7936
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18615
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

Protocols

Product Specific Protocols
WB protocol for RDBP antibody 85945-1-RRDownload protocol
IF protocol for RDBP antibody 85945-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols