Radixin Polyclonal antibody

Radixin Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13790-1-AP

Synonyms

DFNB24, radixin, RDX

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Validation Data Gallery

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  WB analysis of mouse pancreas using 13790-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13790-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13790-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 13790-1-AP

  Immunofluorescent analysis of MCF-7 cells using 13790-1-AP (Radixin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 13790-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Radixin antibody (13790-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, HEK-293 cells, Y79 cells
• Positive IHC detected in: human pancreas cancer tissue, human liver cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13790-1-AP targets Radixin in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Radixin fusion protein Ag4813
• Full Name: radixin
• Calculated Molecular Weight: 583 aa, 68 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC047109
• Gene Symbol: RDX
• Gene ID (NCBI): 5962
• RRID: AB_2300889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Radixin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to chemokine and antigen stimulation.

Publications

Species	Application	Title
mouse	IF	Am J Pathol; Elevated Expression of Moesin in Muscular Dystrophies.; Authors - Mark Pines