CoraLite® Plus 488-conjugated RECQL Recombinant monoclonal antibody

• 

  IF Staining of U-251 using CL488-85024-6

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite® Plus 488 RECQL antibody (CL488-85024-6, Clone: 242577F1 ) at dilution of 1:200, CL594-phalloidin (red).

RECQL Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-85024-6

Clone No.242577F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

242577F1, ATP dependent DNA helicase Q1, ATP-dependent DNA helicase Q1, DNA 3'-5' helicase Q1, DNA dependent ATPase Q1

---

Tested Applications

• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85024-6 targets RECQL in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6834

  Product name: Recombinant human RECQL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 101-448 aa of BC001052

  Sequence: NVTMAGKEVFLVMPTGGGKSLCYQLPALCSDGFTLVICPLISLMEDQLMVLKQLGISATMLNASSSKEHVKWVHAEMVNKNSELKLIYVTPEKIAKSKMFMSRLEKAYEARRFTRIAVDEVHCCSQWGHDFRPDYKALGILKRQFPNASLIGLTATATNHVLTDAQKILCIEKCFTFTASFNRPNLYYEVRQKPSNTEDFIEDIVKLINGRYKGQSGIIYCFSQKDSEQVTVSLQNLGIHAGAYHANLEPEDKTTVHRKWSANEIQVVVATVAFGMGIDKPDVRFVIHHSMSKSMENYYQESGRAGRDDMKADCILYYGFGDIFRISSMVVMENVGQQKLYEMVSYCQ

• Full Name: RecQ protein-like (DNA helicase Q1-like)
• Calculated Molecular Weight: 73 kDa
• GenBank Accession Number: BC001052
• Gene Symbol: RECQL
• Gene ID (NCBI): 5965
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P46063
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RECQL, also known as RecQ-like helicase, is a member of the RecQ DNA helicase family. These enzymes play a crucial role in maintaining genomic integrity by participating in various DNA repair processes, including mismatch repair, nucleotide excision repair, and direct repair. It's reported that the low RECQL expression is strongly associated with poor prognosis in breast cancer. RECQL expression may be a useful marker in estimating the prognosis of breast cancer patients. (PMID: 29914420)