RECQL4 Recombinant monoclonal antibody

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  WB analysis using 86529-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86529-2-RR (RECQL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 86529-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RECQL4 antibody (86529-2-RR, Clone: 251312D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86529-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86529-2-RR against Human RECQL4 were performed. The affinity constant is below 1 pM.

RECQL4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86529-2-RR

Clone No.251312D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ATP dependent DNA helicase Q4, ATP-dependent DNA helicase Q4, DNA 3'-5' helicase RecQ4, DNA helicase, RecQ-like type 4, EC:5.6.2.4

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86529-2-RR targets RECQL4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10707

  Product name: Recombinant human RECQL4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 297-652 aa of BC013277

  Sequence: CTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSHQAAPGPRRVCMGHERALPIQLTVQALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRLNCPGGPAQLQALAHRCPPLAVCLAQQLPEDPGQGSSSVEFDMVKLVDSMGWELASVRRALCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQICDFLYGRVQARERQALARLRRTFQAFHSVAFPSCGPCLEQQDEERSTRLKDLLGRYFEEEEGQEPGGMEDAQGPEPGQARLQDWEDQVRCDIRQFLSLRPEEKFSSRAVARIFHGIGSPCYPAQVYGQDRRFWRKYLHLSFHALVGLATEELLQVAR

• Full Name: RecQ protein-like 4
• Calculated Molecular Weight: 1208 aa, 133 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC013277
• Gene Symbol: RECQL4
• Gene ID (NCBI): 9401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O94761
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.