Tested Applications
| Positive WB detected in | BxPC-3 cells, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | BxPC-3 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, mouse olfactory epithelium tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12088-1-AP targets REEP6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2723 Product name: Recombinant human REEP6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC008201 Sequence: MDGLRQRVEHFLEQRNLVTEVLGALEAKTGVEKRYLAAGAVTLLSLYLLFGYGASLLCNLIGFVYPAYASIKAIESPSKDDDTVWLTYWVVYALFGLAEFFSDLLLSWFPFYYVGKCAFLLFCMAPRPWNGALMLYQRVVRPLFLRHHGAVDRIMNDLSGRALDAAAGITRNVKPSQTPQPKDK Predict reactive species |
| Full Name | receptor accessory protein 6 |
| Calculated Molecular Weight | 201 aa, 22 kDa |
| Observed Molecular Weight | 17-22 kDa |
| GenBank Accession Number | BC008201 |
| Gene Symbol | REEP6 |
| Gene ID (NCBI) | 92840 |
| RRID | AB_2178460 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HR9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for REEP6 antibody 12088-1-AP | Download protocol |
| IHC protocol for REEP6 antibody 12088-1-AP | Download protocol |
| IP protocol for REEP6 antibody 12088-1-AP | Download protocol |
| WB protocol for REEP6 antibody 12088-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
REEP6 Polyclonal antibody (Cat# 12088-1-AP) has 6 total citations published in J Clin Invest, Molecular Psychiatry, International Journal of Biological Macromolecules, American Journal of Human Genetics, Human Molecular Genetics, and bioRxiv. Research fields span neuronal PET imaging, Alzheimer's disease pathology, ferroptosis in oral squamous cell carcinoma, retinitis pigmentosa genetics, retinal degeneration mechanisms, and meganuclease gene editing for inherited retinal disease.
| Species | Application | Title |
|---|---|---|
Mol Psychiatry Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease. | ||
Int J Biol Macromol Receptor accessory protein 6, a novel ferroptosis suppressor, drives oral squamous cell carcinoma by maintaining endoplasmic reticulum hemostasis | ||
Hum Mol Genet REEP6 deficiency leads to retinal degeneration through disruption of ER homeostasis and protein trafficking. | ||
bioRxiv RHO1-2 meganuclease gene editing targets human P23H rhodopsin-induced retinitis pigmentosa to rejuvenate rods and maintain cones. |
