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RELB Recombinant monoclonal antibody, PBS Only (83305-6-PBS)

RELB Recombinant monoclonal antibody, PBS Only

Name: RELB Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 83305-6-PBS-100UG

RELB Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83305-6-PBS

Clone No. 242336G4

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242336G4, I-Rel, Transcription factor RelB

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllWBIF/ICCFC (Intra)Affinity

WB analysis using 83305-6-RR (same clone as 83305-6-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83305-6-RR (RELB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83305-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of THP-1 using 83305-6-RR (same clone as 83305-6-PBS)
Immunofluorescent analysis of (4% PFA) fixed LPS treated THP-1 cells using RELB antibody (83305-6-RR, Clone: 242336G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83305-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Raji using 83305-6-RR (same clone as 83305-6-PBS)
1x10^6 Raji cells were intracellularly stained with 0.25 ug RELB Recombinant antibody (83305-6-RR, Clone:242336G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83305-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 Raji cells were intracellularly stained with 0.25 ug RELB Recombinant antibody (83305-6-RR, Clone:242336G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83305-6-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83305-6-RR
Biolayer interferometry (BLl) kinetic assays of 83305-6-RR against Human RELB were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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Product Information

83305-6-PBS targets RELB in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag13824 Product name: Recombinant human RELB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 264-579 aa of BC028013 Sequence: NVVRICFQASYRDQQGQMRRMDPVLSEPVYDKKSTNTSELRICRINKESGPCTGGEELYLLCDKVQKEDISVVFSRASWEGRADFSQADVHRQIAIVFKTPPYEDLEIVEPVTVNVFLQRLTDGVCSEPLPFTYLPRDHDSYGVDKKRKRGMPDVLGELNSSDPHGIESKRRKKKPAILDHFLPNHGSGPFLPPSALLPDPDFFSGTVSLPGLEPPGGPDLLDDGFAYDPTAPTLFTMLDLLPPAPPHASAVVCSGGAGAVVGETPGPEPLTLDSYQAPGPGDGGTASLVGSNMFPNHYREAAFGGGLLSPGPEAT Predict reactive species
Full Name	v-rel reticuloendotheliosis viral oncogene homolog B
Calculated Molecular Weight	62 kDa
Observed Molecular Weight	62-65 kDa
GenBank Accession Number	BC028013
Gene Symbol	RELB
Gene ID (NCBI)	5971
RRID	AB_3743473
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q01201
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.