Tested Applications
| Positive WB detected in | Raji cells, Daudi cells, MDA-MB-231 cells |
| Positive IF/ICC detected in | LPS treated THP-1 cells |
| Positive FC (Intra) detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83305-6-RR targets RELB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag13824 Product name: Recombinant human RELB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 264-579 aa of BC028013 Sequence: NVVRICFQASYRDQQGQMRRMDPVLSEPVYDKKSTNTSELRICRINKESGPCTGGEELYLLCDKVQKEDISVVFSRASWEGRADFSQADVHRQIAIVFKTPPYEDLEIVEPVTVNVFLQRLTDGVCSEPLPFTYLPRDHDSYGVDKKRKRGMPDVLGELNSSDPHGIESKRRKKKPAILDHFLPNHGSGPFLPPSALLPDPDFFSGTVSLPGLEPPGGPDLLDDGFAYDPTAPTLFTMLDLLPPAPPHASAVVCSGGAGAVVGETPGPEPLTLDSYQAPGPGDGGTASLVGSNMFPNHYREAAFGGGLLSPGPEAT Predict reactive species |
| Full Name | v-rel reticuloendotheliosis viral oncogene homolog B |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 62-65 kDa |
| GenBank Accession Number | BC028013 |
| Gene Symbol | RELB |
| Gene ID (NCBI) | 5971 |
| RRID | AB_3743473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q01201 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RELB antibody 83305-6-RR | Download protocol |
| IF protocol for RELB antibody 83305-6-RR | Download protocol |
| WB protocol for RELB antibody 83305-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RELB Recombinant monoclonal antibody (242336G4), catalog number 83305-6-RR, has 1 total citation. It appears in Cancer Communications (London), in a study on NSUN2-mediated cancer immune evasion and metabolic reprogramming. The cited application is Western blot (WB) in human and mouse samples, placing the research field in oncology and tumor immunology.
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) NSUN2 Promotes Cancer Immune Evasion via Its Moonlighting Function Acting on Metabolic Reprogramming. |
