RELN Polyclonal antibody

RELN Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (2)

Cat no : 20689-1-AP

Synonyms

PRO1598, reelin, RELN, RL

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Validation Data Gallery

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  IHC staining of human liver cancer using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20689-1-AP (RELN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20689-1-AP (RELN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20689-1-AP (RELN Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human liver using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20689-1-AP (RELN Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of mouse brain using 20689-1-AP

  Dual immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20689-1-AP (RELN antibody) at dilution of 1:50 and 66564-1-Ig (Tbr1 antibody), further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 20689-1-AP and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66564-1-Ig.

• 

  IF Staining of mouse brain using 20689-1-AP

  Dual immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20689-1-AP (RELN antibody) at dilution of 1:50 and 66564-1-Ig (Tbr1 antibody), further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 20689-1-AP and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66564-1-Ig.

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Tested Applications

• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

20689-1-AP targets RELN in IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: reelin
• Calculated Molecular Weight: 388 kDa
• GenBank Accession Number: NM_005045
• Gene Symbol: RELN
• Gene ID (NCBI): 5649
• RRID: AB_2878724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RELN belongs to the reelin family. It is an extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. It is a secreted glycoprotein which is essential for neuronal migration in the cortex. RELN regulates microtubule function in neurons and neuronal migration. It affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and ApoER2, RELN induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2).

Publications

Species	Application	Title
pig	IF	Sci Bull (Beijing); Single-cell dynamics of liver development in postnatal pigs; Authors - Lin Rao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

I tried RELN (in red) in human paraffin brain sections and the result was a very good specificity.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

RELN Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:20689-1-AP)

FH

Etienne (Verified Customer) (12-19-2022)

Worked very well at 1/50 and 1/200

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50, 1/200, 1/500
• Cell Tissue Type: Human Brain Organoid

RELN Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoid (Cat no:20689-1-AP)