REPS2 Polyclonal antibody

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  WB analysis using 30670-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30670-1-AP (REPS2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

REPS2 Polyclonal Antibody for WB, ELISA

Cat No. 30670-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

Partner of RalBP1, POB1, RalBP1 interacting protein 2, REPS2

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Tested Applications

• Positive WB detected in: T-47D cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30670-1-AP targets REPS2 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33764

  Product name: Recombinant human REPS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 390-620 aa of NM_004726

  Sequence: LPANQQPRDLNRMEKTSVKDMADLPVPNQDVTSDDKQALKSTINEALPKDVSEDPATPKDSNSLKARPRSRSYSSTSIEEAMKRGEDPPTPPPRPQKTHSRASSLDLNKVFQPSVPATKSGLLPPPPALPPRPCPSQSEQVSEAELLPQLSRAPSQAAESSPAKKDVLYSQPPSKPIRRKFRPENQATENQEPSTAASGPASAATMKPHPTVQKQSSKQKKAIQTAIRKNK

• Full Name: RALBP1 associated Eps domain containing 2
• Calculated Molecular Weight: 72KD
• Observed Molecular Weight: 58 kDa, 78 kDa
• GenBank Accession Number: NM_004726
• Gene Symbol: REPS2
• Gene ID (NCBI): 9185
• RRID: AB_3086383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NFH8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REPS2 is part of a protein complex that regulates the endocytosis of growth factor receptors. The encoded protein directly interacts with a GTPase activating protein that functions downstream of the small G protein Ral. Its expression can negatively affect receptor internalization and inhibit growth factor signaling. There are 4 isoforms of the protein with two short (58 kDa) isoforms comprising 521 or 522 amino acid residues and two long (78 kDa) ones of 659 or 660 residues.