RERG Polyclonal antibody

• Featured Product
• KD/KO Validated

RERG Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10687-1-AP

Synonyms

RERC, RERG

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Validation Data Gallery

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  WB analysis of MCF-7 using 10687-1-AP

  WB result of RERG antibody (10687-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RERG transfected MCF-7 cells.

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  WB analysis using 10687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10687-1-AP (RERG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10687-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10687-1-AP (RERG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10687-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10687-1-AP (RERG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

10687-1-AP targets RERG in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RERG fusion protein Ag1041
• Full Name: RAS-like, estrogen-regulated, growth inhibitor
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23-26 kDa
• GenBank Accession Number: BC007997
• Gene Symbol: RERG
• Gene ID (NCBI): 85004
• RRID: AB_2253772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RERG (Ras-related and estrogen-regulated growth inhibitor), a gene that encodes a small GTP binding and hydrolyzing protein (GTPase) of the Ras superfamily, is a novel gene that was first reported in breast cancer. RERG gene expression is stimulated by the estrogen receptor alpha (ERalpha), and RERG gene expression is absent in ER-negative breast cancer. RERG can bind and hydrolyze GTP as a GTP/GDP molecular switch, and RERG has been shown to possess growth inhibitory activity in breast cancer cells.

Publications

Species	Application	Title
human	WB,IF	Front Oncol; The NFAT3/RERG Complex in Luminal Breast Cancers Is Required to Inhibit Cell Invasion and May Be Correlated With an Absence of Axillary Lymph Nodes Colonization.; Authors - Lucie Coillard ; KD Validated
human	IHC	Breast Cancer Res Treat; RERG (Ras-like, oestrogen-regulated, growth-inhibitor) expression in breast cancer: a marker of ER-positive luminal-like subtype.; Authors - Habashy Hany Onsy HO
		Oncol Rep; ERK5‑regulated RERG expression promotes cancer progression in prostatic carcinoma.; Authors - Ying Xiong
human	WB,IHC	Oncotarget; MicroRNA-382-5p aggravates breast cancer progression by regulating the RERG/Ras/ERK signaling axis.; Authors - Jar-Yi Ho
human	IHC	Front Med (Lausanne); Missing link between tissue specific expressing pattern of ERβ and the clinical manifestations in LGBLEL; Authors - Xujuan Zhang