RFC3 Polyclonal antibody

RFC3 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 11814-1-AP

Synonyms

A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C subunit 3, RF C 38 kDa subunit, RFC3, RFC38



Tested Applications

Positive WB detected inhuman liver tissue, HeLa cells, human heart tissue
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

11814-1-AP targets RFC3 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFC3 fusion protein Ag2269
Full Name replication factor C (activator 1) 3, 38kDa
Calculated molecular weight 40 aa, 6 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000149
Gene symbol RFC3
Gene ID (NCBI) 5983
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase . In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3 and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion and metastasis. RFC3 exists two isoforms with molecular weight of 34 kDa and 38-40 kDa.

Protocols

Product Specific Protocols
WB protocol for RFC3 antibody 11814-1-APDownload protocol
IF protocol for RFC3 antibody 11814-1-APDownload protocol
IP protocol for RFC3 antibody 11814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.

Authors - Stephen Gray
humanWB

Cell Rep

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.

Authors - Jianguo Yang