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RFC3 Polyclonal antibody
RFC3 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IF, ELISA
Cat no : 11814-1-AP
|Positive WB detected in||human liver tissue, HeLa cells, human heart tissue|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11814-1-AP targets RFC3 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RFC3 fusion protein Ag2269|
|Full Name||replication factor C (activator 1) 3, 38kDa|
|Calculated molecular weight||40 aa, 6 kDa|
|Observed molecular weight||38 kDa|
|GenBank accession number||BC000149|
|Gene ID (NCBI)||5983|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase . In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3 and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion and metastasis. RFC3 exists two isoforms with molecular weight of 34 kDa and 38-40 kDa.
|Product Specific Protocols|
|WB protocol for RFC3 antibody 11814-1-AP||Download protocol|
|IF protocol for RFC3 antibody 11814-1-AP||Download protocol|
|IP protocol for RFC3 antibody 11814-1-AP||Download protocol|
|Click here to view our Standard Protocols|
IRF2 inhibits ZIKV replication by promoting FAM111A expression to enhance the host restriction effect of RFC3.
Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.
TRPS1 drives heterochromatic origin refiring and cancer genome evolution.