RFC3 Polyclonal antibody

• Featured Product
• KD/KO Validated

RFC3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11814-1-AP

Synonyms

RF-C 38 kDa subunit

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Validation Data Gallery

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  WB analysis of human liver using 11814-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11814-1-AP (RFC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11814-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11814-1-AP (RFC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11814-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11814-1-AP (RFC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11814-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11814-1-AP (RFC3 antibody) at dilution of 1:300.

• 

  IF Staining of HepG2 using 11814-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11814-1-AP (RFC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human liver tissue, HeLa cells, human heart tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• CoIP: See 1 publications below

Product Information

11814-1-AP targets RFC3 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFC3 fusion protein Ag2269
• Full Name: replication factor C (activator 1) 3, 38kDa
• Calculated Molecular Weight: 40 aa, 6 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000149
• Gene Symbol: RFC3
• Gene ID (NCBI): 5983
• RRID: AB_2178470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase . In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3 and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion and metastasis. RFC3 exists two isoforms with molecular weight of 34 kDa and 38-40 kDa.

Publications

Species	Application	Title
human	WB	Virol J; IRF2 inhibits ZIKV replication by promoting FAM111A expression to enhance the host restriction effect of RFC3.; Authors - Kai Ren ; KD Validated
mouse	WB	Cell Rep; Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.; Authors - Stephen Gray
human	WB	Cell Rep; TRPS1 drives heterochromatic origin refiring and cancer genome evolution.; Authors - Jianguo Yang
human	CoIP	Exp Ther Med; RFC3 drives the proliferation, migration, invasion and angiogenesis of colorectal cancer cells by binding KIF14; Authors - Rong Yu