RFC3 Monoclonal antibody

RFC3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G3A8

Cat no : 68290-1-Ig

Synonyms

A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C subunit 3, RF C 38 kDa subunit, RFC3, RFC38

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Validation Data Gallery

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  WB analysis using 68290-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of NIH/3T3 using 68290-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 68290-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68290-1-Ig targets RFC3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RFC3 fusion protein Ag28365
• Full Name: replication factor C (activator 1) 3, 38kDa
• Calculated Molecular Weight: 40 aa, 6 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000149
• Gene Symbol: RFC3
• Gene ID (NCBI): 5983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in the nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase. In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair, and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3, and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion, and metastasis. RFC3 exists in two isoforms with molecular weights of 34 kDa and 38-40 kDa.