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RFTN1 Polyclonal antibody, PBS Only (24289-1-PBS)

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WB analysis of HeLa using 24289-1-AP (same clone as 24289-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 24289-1-AP (RFTN1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 24289-1-AP (same clone as 24289-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 24289-1-AP (RFTN1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 24289-1-AP (same clone as 24289-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 24289-1-AP (same clone as 24289-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

RFTN1 Polyclonal antibody, PBS Only

Name: RFTN1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 24289-1-PBS

RFTN1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 24289-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Raftlin, PIG9, PIB10, MIG2, KIAA0084

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

24289-1-PBS targets RFTN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17264 Product name: Recombinant human RFTN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 29-374 aa of BC100667 Sequence: ETKIDVSYEYRFLEFTTLSAAELPGSSAVRLASLRDLPAQLLELYQQGFSLAALHPFVQPTHEREKTPLEHIFRAILIKKTDRSQKTDLHNEGYILELDCCSSLDHPTDQKLIPEFIKKIQEAASQGLKFVGVIPQYHSSVNSAGSSAPVSTANSTEDARDAKNARGDHASLENEKPGTGDVCSAPAGRNQSPEPSSGPRGEVPLAKQPSSPSGEGDGGELSPQGVSKTLDGPESNPLEVHEEPLSGKMEIFTLFNKPKSHQKCRQYYPVTIPLHVSKNGQTVSGLDANWLEHMSDHFRKGGMLVNAVFYLGIVNDSLHGLTDGVFIFEAVSTEDSKTIQGYDAIV Predict reactive species
Full Name	raftlin, lipid raft linker 1
Calculated Molecular Weight	578 aa, 63 kDa
Observed Molecular Weight	63-70 kDa
GenBank Accession Number	BC100667
Gene Symbol	RFTN1
Gene ID (NCBI)	23180
RRID	AB_2879474
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14699
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Raftlin (RFTN1) was originally identified as a major raft protein with molecular mass of 63 kDa in B cells that colocalized with BCR in the lipid raft before and after BCR activation. RFTN1 possesses fatty acylation sites at the N terminus, it predominantly localizes in the cytoplasm in human epithelial cells and monocyte-derived DCs (Mo-DCs) (PMID: 27022195). Human RFTN1 protein estimated to be 63~70 kDa bySDS-PAGE, and this discrepancy may be due to the acidic nature of the protein, which has a calculated isoelectric point of 5.3 (PMID: 12805216).