RGC32 Polyclonal antibody, PBS Only

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• RGC32 Polyclonal antibody, PBS Only (19556-1-PBS)

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  WB analysis using 19556-1-AP (same clone as 19556-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19556-1-AP (RGC32 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19556-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 19556-1-AP (same clone as 19556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19556-1-AP (RGC32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19556-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 19556-1-AP (same clone as 19556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19556-1-AP (RGC32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19556-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 19556-1-AP (same clone as 19556-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using RGC32 antibody (19556-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 19556-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RGC32 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 19556-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C13orf15, bA157L14.2, RGCC, RGC-32, RGC 32

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Product Information

19556-1-PBS targets RGC32 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: chromosome 13 open reading frame 15
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 13-15 kDa
• GenBank Accession Number: NM_014059
• Gene Symbol: RGC32
• Gene ID (NCBI): 28984
• RRID: AB_3085580
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H4X1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RGC32, also named as C13orf15, modulates the activity of cell cycle-specific kinases. RGC32 enhances CDC2 activity. RGC32 may contribute to the regulation of the cell cycle. And it inhibits growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. The antibody can recognize these 2 isoforms of RGC32.