RGS7 Polyclonal antibody

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• RGS7 Polyclonal antibody (14056-1-AP)

RGS7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14056-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

regulator of G protein signaling 7, Regulator of G-protein signaling 7

• 

  WB analysis of SH-SY5Y using 14056-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14056-1-AP (RGS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SH-SY5Y using 14056-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14056-1-AP (RGS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 14056-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14056-1-AP (RGS7 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 14056-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14056-1-AP (RGS7 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of SH-SY5Y using 14056-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using RGS7 antibody (14056-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14056-1-AP targets RGS7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4982

  Product name: Recombinant human RGS7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 139-487 aa of BC022009

  Sequence: NKARLELADYEAESLARLQRAFARKWEFIFMQAEAQAKVDKKRDKIERKILDSQERAFWDVHRPVPGCVNTTEVDIKKSSRMRNPHKTRKSVYGLQNDIRSHSPTHTPTPETKPPTEDELQQQIKYWQIQLDRHRLKMSKVADSLLSYTEQYLEYDPFLLPPDPSNPWLSDDTTFWELEASKEPSQQRVKRWGFGMDEALKDPVGREQFLKFLESEFSSENLRFWLAVEDLKKRPIKEVPSRVQEIWQEFLAPGAPSAINLDSKSYDKTTHNVKEPGRYTFEDAQEHIYKLMKSDSYPRFIRSSAYQELLQAKKKSGNSMDRRTSFEKFAQNVGKSLTSKRLTSLAQSY

• Full Name: regulator of G-protein signaling 7
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58-62 kDa
• GenBank Accession Number: BC022009
• Gene Symbol: RGS7
• Gene ID (NCBI): 6000
• RRID: AB_2238316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49802
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Regulator of G-protein signaling 7 (RGS7) expression is both necessary and sufficient to promote doxorubicin-dependent acetylation and activation of NF-κB subunit p65 and resultant production of pro-inflammatory cytokines from cardiomyocytes (PMID: 37589751). RGS7 promotes apoptosis in cardiac myocytes by increasing oxidative stress and impairing mitochondrial function, actions that involve a direct and functional interaction between RGS7 and the Ca2+/calmodulin-dependent kinase CaMKII (PMID: 36574707). In non-neuronal cell types, it is possible that a greater proportion of RGS7 may be present in the cytosol where it is free to interact with additional effectors (PMID: 36574707).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IHC	Biochim Biophys Acta Mol Basis Dis; Silencing RGS7 attenuates atrial fibrillation progression by activating the cGMP-PKG signaling pathway; Authors - Hao Huang
		Epigenomics; Methylation changes of gallbladder DNA during the formation of gallstones.; Authors - Hongyu Xu