Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12467-1-AP targets RHBDL2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3142 Product name: Recombinant human RHBDL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-132 aa of BC013103 Sequence: MAAVHDLEMESMNLNMGREMKEELEEEEKMREDGGGKDRAKSKKVHRIVSKWMLPEKSRGTYLERANCFPPPVFIISISLAELAVFIYYAVWKPQKQWITLDTGILESPFIYSPEKREEAWRFISYMLVHAG Predict reactive species |
| Full Name | rhomboid, veinlet-like 2 (Drosophila) |
| Calculated Molecular Weight | 303 aa, 34 kDa |
| Observed Molecular Weight | 34-37 kDa |
| GenBank Accession Number | BC013103 |
| Gene Symbol | RHBDL2 |
| Gene ID (NCBI) | 54933 |
| RRID | AB_11232403 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NX52 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RHBDL2 antibody 12467-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-RHBDL2 (catalog #12467-1-AP) has 9 citations published in Molecular Cell, Cell Death & Disease, Journal of Cell Biology, International Journal of Molecular Sciences, Scientific Reports, Biophysical Journal, and Cellular and Molecular Life Sciences. Research fields span CRAC channel regulation, lipid metabolic reprogramming in osteosarcoma, pancreatic cancer via Notch signaling, BACE1/γ-secretase processing, cadherin regulation, epithelial homeostasis, renal cell carcinoma, membrane protein dimerization, and EGFR signaling in keratinocytes.
| Species | Application | Title |
|---|---|---|
Mol Cell Conformational surveillance of Orai1 by a rhomboid intramembrane protease prevents inappropriate CRAC channel activation.
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Cell Death Dis RHBDL2 promotes the proliferation, migration, and invasion of pancreatic cancer by stabilizing the N1ICD via the OTUD7B and activating the Notch signaling pathway
| ||
Cell Death Dis RHBDL2 drives lipid metabolic reprogramming in osteosarcoma via USP3-mediated deubiquitination of PPT1.
| ||
J Cell Biol A cellular complex of BACE1 and γ-secretase sequentially generates Aβ from its full-length precursor. | ||
Int J Mol Sci Rhomboid-Like-2 Intramembrane Protease Mediates Metalloprotease-Independent Regulation of Cadherins.
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Sci Rep Rhomboid-like 2 correlated with TME infiltration inhibits cuproptosis-related genes and drives malignant phenotype in clear cell renal cell carcinoma |
