RHBDL2 Polyclonal antibody

• 

  WB analysis of HeLa using 12467-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12467-1-AP (RHBDL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

RHBDL2 Polyclonal Antibody for WB, ELISA

Cat No. 12467-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

RHBDL2, Rhomboid like protein 2, Rhomboid related protein 2, RRP2

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12467-1-AP targets RHBDL2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3142

  Product name: Recombinant human RHBDL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-132 aa of BC013103

  Sequence: MAAVHDLEMESMNLNMGREMKEELEEEEKMREDGGGKDRAKSKKVHRIVSKWMLPEKSRGTYLERANCFPPPVFIISISLAELAVFIYYAVWKPQKQWITLDTGILESPFIYSPEKREEAWRFISYMLVHAG

• Full Name: rhomboid, veinlet-like 2 (Drosophila)
• Calculated Molecular Weight: 303 aa, 34 kDa
• Observed Molecular Weight: 34-37 kDa
• GenBank Accession Number: BC013103
• Gene Symbol: RHBDL2
• Gene ID (NCBI): 54933
• RRID: AB_11232403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NX52
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; Conformational surveillance of Orai1 by a rhomboid intramembrane protease prevents inappropriate CRAC channel activation.; Authors - Adam G Grieve ; KO Validated
	WB	J Cell Biol; A cellular complex of BACE1 and γ-secretase sequentially generates Aβ from its full-length precursor.; Authors - Lei Liu
human	WB	Sci Rep; Rhomboid-like 2 correlated with TME infiltration inhibits cuproptosis-related genes and drives malignant phenotype in clear cell renal cell carcinoma; Authors - Zhifei Che
human	WB	Sci Rep; Quantitative proteomics screen identifies a substrate repertoire of rhomboid protease RHBDL2 in human cells and implicates it in epithelial homeostasis.; Authors - Nicholas Johnson ; KD Validated
human	WB,IF	Int J Mol Sci; Rhomboid-Like-2 Intramembrane Protease Mediates Metalloprotease-Independent Regulation of Cadherins.; Authors - Chiara Battistini ; KD Validated
human	WB	Biophys J; Membrane Protein Dimerization in Cell-Derived Lipid Membranes Measured by FRET with MC Simulations.; Authors - Jan Škerle ; KD Validated