RHBDL3 Polyclonal antibody

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  WB analysis using 21671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21671-1-AP (RHBDL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

RHBDL3 Polyclonal Antibody for WB, ELISA

Cat No. 21671-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21671-1-AP targets RHBDL3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RHBDL3 fusion protein Ag16499
• Full Name: rhomboid, veinlet-like 3 (Drosophila)
• Calculated Molecular Weight: 404 aa, 45 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC100975
• Gene Symbol: RHBDL3
• Gene ID (NCBI): 162494
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P58872
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RHBDL3 (Rhomboid-related protein 3) is involved in a process known as regulated intramembrane proteolysis (RIP), where proteins are cleaved within the membrane to release functional polypeptides from their membrane anchors. It is expressed in several structures, including the central nervous system, embryo ectoderm, reproductive system, sensory organ, and thymus. RHBDL3 is also a prognostic marker in Glioblastoma multiforme, indicating its potential role in cancer-related pathways. It is expressed in various cell lines, particularly in those related to neuronal function and visual perception, with low cancer specificity.