• Featured Product
  • KD/KO Validated

RHEB Polyclonal antibody

RHEB Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 15924-1-AP


GTP binding protein Rheb, Ras homolog enriched in brain, RHEB, RHEB2

Tested Applications

Positive WB detected infetal human brain tissue, 3T3-L1 cells, DU 145 cells, HeLa cells, human brain tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman pancreas cancer tissue, mouse pancreas tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15924-1-AP targets RHEB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHEB fusion protein Ag8747
Full Name Ras homolog enriched in brain
Calculated molecular weight 184 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC016155
Gene symbol RHEB
Gene ID (NCBI) 6009
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.


Product Specific Protocols
WB protocol for RHEB antibody 15924-1-APDownload protocol
IHC protocol for RHEB antibody 15924-1-APDownload protocol
IF protocol for RHEB antibody 15924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Metab

Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.

Authors - Noriaki Shimizu

PLoS Biol

RHEB/mTOR hyperactivity causes cortical malformations and epileptic seizures through increased axonal connectivity.

Authors - Martina Proietti Onori


Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression.

Authors - Yan Yang
  • KO Validated

Front Cell Dev Biol

PPT1 Reduction Contributes to Erianin-Induced Growth Inhibition in Oral Squamous Carcinoma Cells.

Authors - Qingqiong Luo

Stem Cell Res Ther

Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.

Authors - Caixia Jin

Exp Mol Med

A brain somatic RHEB doublet mutation causes focal cortical dysplasia type II.

Authors - Shanshan Zhao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain