CoraLite® Plus 488-conjugated RHOA Recombinant antibody

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  IF Staining of HeLa using CL488-82760-2

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RHOA antibody (CL488-82760-2, Clone: 240880E5 ) at dilution of 1:200.

• Featured Product
• KD/KO Validated

RHOA Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-82760-2

Clone No.240880E5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

Synonyms

ARH12, ARHA, RHO12, 240880E5, EC:3.6.5.2

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82760-2 targets RHOA in applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RHOA fusion protein Ag1141
• Full Name: ras homolog gene family, member A
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC005976
• Gene Symbol: RHOA
• Gene ID (NCBI): 387
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61586
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.