RHOC Monoclonal antibody

RHOC Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1G1C1

Cat no : 67542-1-Ig

Synonyms

ARH9, ARHC, H9, Rho cDNA clone 9, RHOC, RHOH9



Tested Applications

Positive WB detected inLNCaP cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, C6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, RAW264.7 cells
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67542-1-Ig targets RHOC in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RHOC fusion protein Ag14633
Full Name ras homolog gene family, member C
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC007245
Gene symbol RHOC
Gene ID (NCBI) 389
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Ras homolog family member C (RHOC) encodes a Rho-related GTP-binding protein, which belongs to small GTPase superfamily and also Rho family. RHOC. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas and breast cancer as well.

Protocols

Product Specific Protocols
WB protocol for RHOC antibody 67542-1-IgDownload protocol
IF protocol for RHOC antibody 67542-1-IgDownload protocol
FC protocol for RHOC antibody 67542-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols