RHOD Polyclonal antibody

RHOD Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11717-1-AP

Synonyms

ARHD, Rho, Rho related protein HP1, RHOD, RHOHP1, RHOM

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Validation Data Gallery

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  IHC staining of human lung cancer using 11717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11717-1-AP (RHOD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11717-1-AP (RHOD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 11717-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11717-1-AP (RHOD Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 11717-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11717-1-AP (RHOD Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of Human Retina Whole Mount using 11717-1-AP

  Human Retina Whole Mount generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for RHOD with 11717-1-AP at 1:400, ARR3 1:400 Nuclear stain DAPI (blue). Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

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Tested Applications

• Positive IHC detected in: human lung cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Human Retina Whole Mount

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11717-1-AP targets RHOD in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RHOD fusion protein Ag2291
• Full Name: ras homolog gene family, member D
• Calculated Molecular Weight: 210 aa, 23 kDa
• GenBank Accession Number: BC001338
• Gene Symbol: RHOD
• Gene ID (NCBI): 29984
• RRID: AB_2877789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RhoD is a member of the Rho family of small GTPases, and it has been implicated in integrating actin reorganization and endosome motility. RhoD localizes to early endosomes and recycling endosomes, which indicates its important role in the regulation of endosome trafficking. It is involved in reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton.