RIC8A Polyclonal antibody

RIC8A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11138-1-AP

Synonyms

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A

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Validation Data Gallery

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  WB analysis using 11138-1-AP

  BxPC-3 cells and Jurkat cells were subjected to SDS PAGE followed by western blot with 11138-1-AP (RIC8A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11138-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11138-1-AP (RIC8A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11138-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11138-1-AP (RIC8A antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, human brain tissue, Jurkat cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11138-1-AP targets RIC8A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIC8A fusion protein Ag1593
• Full Name: resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 58-62 kDa
• GenBank Accession Number: BC011821
• Gene Symbol: RIC8A
• Gene ID (NCBI): 60626
• RRID: AB_2179822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation.; Authors - Yunfan Yang
mouse	WB,IHC	Front Cell Neurosci; Ric-8A, a Galpha protein guanine nucleotide exchange factor potentiates taste receptor signaling.; Authors - Fenech Claire C