|Positive WB detected in||NCI-H1299 cells, HeLa cells, HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Sample-dependent, check data in validation data gallery|
66867-2-Ig targets RICTOR in WB,ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||RICTOR fusion protein Ag28487|
|Full Name||rapamycin-insensitive companion of mTOR|
|Calculated molecular weight||192 kDa|
|Observed molecular weight||192 kDa|
|GenBank accession number||BC029608|
|Gene ID (NCBI)||253260|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RICTOR, is a key component of the mTOR complex 2 (mTORC2) and is required for phosphorylation of Akt at serine 473. RICTOR is the upstream kinase of several AGC kinase family members including AKT, SGK, S6K mutants and several PKC isoforms. Activation of RICTOR-mTORC2 modifies actin organization and promotes cell proliferation and survival. Rictor is overexpressed in several cancers leading to hyperactive mTORC2 and has been shown to play a causal role in glioma formation. Rictor expression has been demonstrated to be regulated transcriptionally and via protein degradation.