RICTOR Monoclonal antibody

RICTOR Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1D1C4

Cat no : 66867-2-Ig

Synonyms

AVO3 homolog, DKFZp686B11164, hAVO3, KIAA1999, mAVO3, RICTOR



Tested Applications

Positive WB detected inNCI-H1299 cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66867-2-Ig targets RICTOR in WB,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RICTOR fusion protein Ag28487
Full Name rapamycin-insensitive companion of mTOR
Calculated molecular weight 192 kDa
Observed molecular weight 192 kDa
GenBank accession numberBC029608
Gene symbol RICTOR
Gene ID (NCBI) 253260
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RICTOR, is a key component of the mTOR complex 2 (mTORC2) and is required for phosphorylation of Akt at serine 473. RICTOR is the upstream kinase of several AGC kinase family members including AKT, SGK, S6K mutants and several PKC isoforms. Activation of RICTOR-mTORC2 modifies actin organization and promotes cell proliferation and survival. Rictor is overexpressed in several cancers leading to hyperactive mTORC2 and has been shown to play a causal role in glioma formation. Rictor expression has been demonstrated to be regulated transcriptionally and via protein degradation.

Protocols

Product Specific Protocols
WB protocol for RICTOR antibody 66867-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Mol Biosci

FLCN Regulates HIF2α Nuclear Import and Proliferation of Clear Cell Renal Cell Carcinoma.

Authors - Xuyang Zhao