RILP Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 82996-4-RR (same clone as 82996-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82996-4-RR (RILP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 82996-4-RR (same clone as 82996-4-PBS)

  WB result of RILP antibody (82996-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILP transfected HepG2 cells. This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82996-4-RR (same clone as 82996-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82996-4-RR (same clone as 82996-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82996-4-RR (same clone as 82996-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RILP Recombinant antibody (82996-4-RR, Clone:230208G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00025-1

  Cytometric bead array standard curve of MP00025-1, RILP Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82996-4-PBS. Detection antibody: 82996-3-PBS. Standard: Ag4472. Range: 1.25-20 ng/mL.

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  Affinity and Kinetic Characterization of 82996-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82996-4-RR against Human IGFBP4 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

RILP Uni-rAb^® Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 82996-4-PBS

Clone No.230208G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

230208G2, FLJ31193, PP10141, Rab-interacting lysosomal protein

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Product Information

82996-4-PBS targets RILP as part of a matched antibody pair:

MP00025-1: 82996-4-PBS capture and 82996-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Affinity:
  K_D=1.04 x 10^-11M
  K_Off=2.33 x 10^-5M
  K_On=2.05 x 10^6M
• Immunogen:

  CatNo: Ag4472

  Product name: Recombinant human RILP protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 102-401 aa of BC031621

  Sequence: LRAGPQEERALLRQLKEVTDRQRDELRAHNRDLRQRGQETEALQEQLQRLLLVNAELRHKLAAMQTQLRAAQDRERERQQPGEAATPQAKERARGQAGRPGHQHGQEPEWATAGAGAPGNPEDPAEAAQQLGRPSEAGQCRFSREEFEQILQERNELKAKVFLLKEELAYFQRELLTDHRVPGLLLEAMKVAVRKQRKKIKAKMLGTPEEAESSEDEAGPWILLSDDKGDHPPPPESKIQSFFGLWYRGKAESSEDETSSPAPSKLGGEEEAQPQSPAPDPPCSALHEHLCLGASAAPEA

• Full Name: Rab interacting lysosomal protein
• Calculated Molecular Weight: 401 aa, 41 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC031621
• Gene Symbol: RILP
• Gene ID (NCBI): 83547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96NA2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.