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  • Western Blot (WB) analysis of various lysates using RILP Recombinant antibody (82996-4-RR)

    WB analysis using 82996-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82996-4-RR (RILP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RILP Recombinant antibody (82996-4-RR)

    WB analysis of HepG2 using 82996-4-RR

    WB result of RILP antibody (82996-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILP transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using RILP Recombinant antibody (82996-4-RR)

    IHC staining of human ovarian cancer using 82996-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovarian cancer using RILP Recombinant antibody (82996-4-RR)

    IHC staining of human ovarian cancer using 82996-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using RILP Recombinant antibody (82996-4-RR)

    FC experiment of HeLa using 82996-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RILP Recombinant antibody (82996-4-RR, Clone:230208G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82996-4-RR

    Affinity and Kinetic Characterization of 82996-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82996-4-RR against Human IGFBP4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

RILP Recombinant antibody

RILP Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Cat No. 82996-4-RR
Clone No.230208G2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

230208G2, FLJ31193, PP10141, Rab-interacting lysosomal protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 750
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82996-4-RR targets RILP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=1.04 x 10-11M
KOff=2.33 x 10-5M
KOn=2.05 x 106M
Immunogen RILP fusion protein Ag4472 Predict reactive species
Full Name Rab interacting lysosomal protein
Calculated Molecular Weight 401 aa, 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC031621
Gene Symbol RILP
Gene ID (NCBI) 83547
RRIDAB_3670743
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96NA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RILP antibody 82996-4-RRDownload protocol
IHC protocol for RILP antibody 82996-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols