RILPL1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 83220-6-RR (same clone as 83220-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83220-6-RR (RILPL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83220-6-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 83220-6-RR (same clone as 83220-6-PBS)

  WB result of RILPL1 antibody (83220-6-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILPL1 transfected HepG2 cells. This data was developed using the same antibody clone with 83220-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83220-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83220-6-RR against Human RILPL1 were performed. The affinity constant is 1.97 nM.

• Featured Product
• KD/KO Validated

RILPL1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83220-6-PBS

Clone No.230515D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RLP1, RILP-like protein 1, RILP like protein 1, Rab-interacting lysosomal-like protein 1, FLJ39378

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Product Information

83220-6-PBS targets RILPL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10192

  Product name: Recombinant human RILPL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-252 aa of BC080626

  Sequence: MTGMSERERQVMKKLKEVVDKQRDEIRAKDRELGLKNEDVEALQQQQTRLMKINHDLRHRVTVVEAQGKALIEQKVELEADLQTKEQEMGSLRAELGKLRERLQGEHSQNGEEEPETEPVGEESISDAEKVAMDLKDPNRPRFTLQELRDVLHERNELKSKVFLLQEELAYYKSEEMEEENRIPQPPPIAHPRTSPQPESGIKRLFSFFSRDKKRLANTQRNVHIQESFGQWANTHRDDGYTEQGQEALQHL

• Full Name: Rab interacting lysosomal protein-like 1
• Calculated Molecular Weight: 252 aa, 29 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC080626
• Gene Symbol: RILPL1
• Gene ID (NCBI): 353116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5EBL4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Rab effector-related proteins Rab-interacting lysosomal protein-like 1 (Rilpl1) and Rilpl2 regulate protein localization in the primary cilium. Rilpl2 was initially identified as up-regulated in ciliating mouse tracheal epithelial cells. Rilpl1 and Rilpl2 both localize to the primary cilium and centrosome, Rilpl1 specifically to the distal end of the mother centriole.(PMID:23264467)