Tested Applications
| Positive WB detected in | A549 cells, U-118 MG cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16388-1-AP targets RIN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9451 Product name: Recombinant human RIN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 434-783 aa of BC014417 Sequence: KSLHCSVLKPLRPILAARLRRRLAADGSLGRLAEGLRLARAQGPGAFGSHLSLPSPVELEQVRQKLLQLLRTYSPSAQVKRLLQACKLLYMALRTQEGEGAGADEFLPLLSLVLAHCDLPELLLEAEYMSELLEPSLLTGEGGYYLTSLSASLALLSGLGQAHTLPLSPVQELRRSLSLWEQRRLPATHCFQHLLRVAYQDPSSGCTSKTLAVPPEASIATLNQLCATKFRVTQPNTFGLFLYKEQGYHRLPPGALAHRLPTTGYLVYRRAEWPETQGAVTEEEGSGQSEARSRGEEQGCQGDGDAGVKASPRDIREQSETTAEGGQGQAQEGPAQPGEPEAEGSRAAEE Predict reactive species |
| Full Name | Ras and Rab interactor 1 |
| Calculated Molecular Weight | 783 aa, 84 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC014417 |
| Gene Symbol | RIN1 |
| Gene ID (NCBI) | 9610 |
| RRID | AB_2177839 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13671 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RIN1 antibody 16388-1-AP | Download protocol |
| IP protocol for RIN1 antibody 16388-1-AP | Download protocol |
| WB protocol for RIN1 antibody 16388-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RIN1 Polyclonal antibody (Cat# 16388-1-AP) has 3 citations published in Cell Communications and Signaling, Computational and Structural Biotechnology Journal, and Molecular Pain. The research fields span hepatocellular carcinoma signaling (PI3K-AKT pathway), structure-based drug discovery targeting Rab5 for cancer suppression, and TRPV1-dependent pain sensitization in bone cancer models. Applications cited include WB, IHC, IF, and IP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Commun Signal Circular dorsal ruffles disturb the growth factor-induced PI3K-AKT pathway in hepatocellular carcinoma Hep3B cells. | ||
Comput Struct Biotechnol J Structure-based discovery of neoandrographolide as a novel inhibitor of Rab5 to suppress cancer growth. | ||
Mol Pain EXPRESS: RIN1 inhibited TRPV1-dependent pain sensitization in a mouse model of bone cancer pain.
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Reviews
What customers say
One reviewer (Annabel M.) gave a 5-star rating for Western Blot on B cells using lot 00007775 at a 1:1000 dilution. The antibody produced a clear band at ~90 KDa, consistent with expectations and confirmed by mass spectrometry proteomics, though some higher non-specific bands were observed.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Annabel (Verified Customer) (03-17-2026) | Produced a clear band at ~90KDa, results were as expected and confirmed by mass spec proteomics, although there were some higher non-specific bands.
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