Tested Applications
| Positive WB detected in | A549 cells, U-118 MG cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16388-1-AP targets RIN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9451 Product name: Recombinant human RIN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 434-783 aa of BC014417 Sequence: KSLHCSVLKPLRPILAARLRRRLAADGSLGRLAEGLRLARAQGPGAFGSHLSLPSPVELEQVRQKLLQLLRTYSPSAQVKRLLQACKLLYMALRTQEGEGAGADEFLPLLSLVLAHCDLPELLLEAEYMSELLEPSLLTGEGGYYLTSLSASLALLSGLGQAHTLPLSPVQELRRSLSLWEQRRLPATHCFQHLLRVAYQDPSSGCTSKTLAVPPEASIATLNQLCATKFRVTQPNTFGLFLYKEQGYHRLPPGALAHRLPTTGYLVYRRAEWPETQGAVTEEEGSGQSEARSRGEEQGCQGDGDAGVKASPRDIREQSETTAEGGQGQAQEGPAQPGEPEAEGSRAAEE Predict reactive species |
| Full Name | Ras and Rab interactor 1 |
| Calculated Molecular Weight | 783 aa, 84 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC014417 |
| Gene Symbol | RIN1 |
| Gene ID (NCBI) | 9610 |
| RRID | AB_2177839 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13671 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RIN1 antibody 16388-1-AP | Download protocol |
| IP protocol for RIN1 antibody 16388-1-AP | Download protocol |
| WB protocol for RIN1 antibody 16388-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RIN1 Polyclonal antibody (Cat# 16388-1-AP) has 3 citations. They appear in Cell Communication and Signaling, Computational and Structural Biotechnology Journal, and Molecular Pain. Research fields span hepatocellular carcinoma and the PI3K-AKT signaling pathway, structure-based drug discovery targeting Rab5 in cancer, and TRPV1-dependent pain sensitization in bone cancer models. Applications cited include WB, IP, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Commun Signal Circular dorsal ruffles disturb the growth factor-induced PI3K-AKT pathway in hepatocellular carcinoma Hep3B cells. | ||
Comput Struct Biotechnol J Structure-based discovery of neoandrographolide as a novel inhibitor of Rab5 to suppress cancer growth. | ||
Mol Pain EXPRESS: RIN1 inhibited TRPV1-dependent pain sensitization in a mouse model of bone cancer pain.
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Reviews
What customers say
The single review for this product (rated 5/5) reports successful use in Western Blot on B cells at a 1:1000 dilution. The antibody produced a clear band at the expected ~90 kDa, with results further confirmed by mass spectrometry proteomics. The reviewer noted some higher molecular weight non-specific bands but overall found the performance satisfactory and as expected.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Annabel (Verified Customer) (03-17-2026) | Produced a clear band at ~90KDa, results were as expected and confirmed by mass spec proteomics, although there were some higher non-specific bands.
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