RIN2 Polyclonal antibody

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  WB analysis using 31223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31223-1-AP (RIN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

RIN2 Polyclonal Antibody for WB, ELISA

Cat No. 31223-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Ras and Rab interactor 2, Ras inhibitor JC265, RASSF4

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, LNCaP cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31223-1-AP targets RIN2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIN2 fusion protein Ag35011
• Full Name: Ras and Rab interactor 2
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: NM_018993
• Gene Symbol: RIN2
• Gene ID (NCBI): 54453
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WYP3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIN2 (Ras and Rab interactor 2), also known as RASSF4. RIN2 is mainly located in Golgi apparatus and participates in the process of material transport and secretion in cells. It is also distributed in cytoplasmic matrix, which may be related to signal transduction in cells. It is highly expressed in the brain, and also in the cerebellum, spinal cord and other nervous system tissues. RIN2 belongs to the family of Ras and Rab exchange factors and has a unique VPS9 domain. It can be used as a nucleotide exchange factor to activate the downstream small GTP enzymes Rab5 and R-Ras, thus starting intracellular signal transduction and regulating cytoskeleton recombination, synapse secretion and cell adhesion. The molecular weight of RIN2 is 100 kDa.