RIOK3 Polyclonal antibody, PBS Only

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• RIOK3 Polyclonal antibody, PBS Only (13593-1-PBS)

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  WB analysis using 13593-1-AP (same clone as 13593-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 13593-1-AP (same clone as 13593-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 13593-1-AP (same clone as 13593-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13593-1-AP (same clone as 13593-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13593-1-AP (same clone as 13593-1-PBS)

  IP result of anti-RIOK3 (IP:13593-1-AP, 4ug; Detection:13593-1-AP 1:600) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13593-1-AP (same clone as 13593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13593-1-AP (same clone as 13593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13593-1-AP (same clone as 13593-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RIOK3 antibody (13593-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 13593-1-AP (same clone as 13593-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RIOK3 antibody (13593-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RIOK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 13593-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

sudD homolog, SUDD, Serine/threonine-protein kinase RIO3, RIO kinase 3 (yeast), RIO kinase 3

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Product Information

13593-1-PBS targets RIOK3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4497

  Product name: Recombinant human RIOK3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 215-516 aa of BC039729

  Sequence: RRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE

• Full Name: RIO kinase 3 (yeast)
• Calculated Molecular Weight: 516 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC039729
• Gene Symbol: RIOK3
• Gene ID (NCBI): 8780
• RRID: AB_2178105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14730
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RIOK3(Serine/threonine-protein kinase RIO3) is initially characterized as a homolog of Aspergillus nidulans sudD and showed down-regulation at the invasive front of malignant melanomas.On Western blots, human RIOK3 migrated as a 60-kD protein. Northern blot analysis indicated that the approximately 4-kb RIOK3 mRNA is expressed widely in human tissues, with additional smaller transcripts present in testis.