Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lymphoma tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11277-1-AP targets RMP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1789 Product name: Recombinant human RMP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 125-448 aa of BC026184 Sequence: MSDAAGDIVDIREEIKCDFEFKAKHRIAHKPHSKPKTSDIFEADIANDVKSKDLLADKELWARLEELERQEELLGELDSKPDTVIANGEDTTSSEEEKEDRNTNVNAMHQVTDSHTPCHKDVASSEPFSGQVNSQLNCSVNGSSSYHSDDDDDDDDDDDDDNIDDDDGDNDHEALGVGDNSIPTIYFSHTVEPKRVRINTGKNTTLKFSEKKEEAKRKRKNSTGSGHSAQELPTIRTPADIYRAFVDVVNGEYVPRKSILKSRSRENSVCSDTSESSAAEFDDRRGVLRSISCEEATCSDTSESILEEEPQENQKKLLPLSVT Predict reactive species |
| Full Name | chromosome 19 open reading frame 2 |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC026184 |
| Gene Symbol | RMP |
| Gene ID (NCBI) | 8725 |
| RRID | AB_10596929 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94763 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RMP antibody 11277-1-AP | Download protocol |
| IP protocol for RMP antibody 11277-1-AP | Download protocol |
| WB protocol for RMP antibody 11277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-URI/RMP antibody (Cat# 11277-1-AP) has 9 citations published in journals including Nature Communications, Cardiovascular Research, Cell Death & Disease, Oncogene, Cell Communication and Signaling, Oxidative Medicine and Cellular Longevity, Inflammation Research, Oncotarget, and American Journal of Cancer Research. Research fields span liver cancer ferroptosis, heart failure, multiple myeloma, innate immunity in sepsis, hepatocellular carcinoma metastasis, myocardial infarction, kidney fibrosis, and gastric cancer.
| Species | Application | Title |
|---|---|---|
Nat Commun URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers
| ||
Cardiovasc Res Cardiomyocyte-specific loss of RMP causes myocardial dysfunction and heart failure. | ||
Cell Death Dis URI regulates tumorigenicity and chemotherapeutic resistance of multiple myeloma by modulating IL-6 transcription.
| ||
Cell Commun Signal RNA polymerase II subunit 5-mediating protein limits TLR4-induced innate immune activation in macrophages by inhibiting IKKβ/NF-κB signaling during sepsis
| ||
Oncogene RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription.
| ||
Oxid Med Cell Longev Macrophage Rmp Ameliorates Myocardial Infarction by Modulating Macrophage Polarization in Mice |
