Tested Applications
| Positive IHC detected in | human liver tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16425-1-AP targets RNASE6 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9667 Product name: Recombinant human RNASE6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-150 aa of BC020848 Sequence: WPKRLTKAHWFEIQHIQPSPLQCNRAMSGINNYTQHCKHQNTFLHDSFQNVAAVCDLLSIVCKNRRHNCHQSSKPVNMTDCRLTSGKYPQCRYSAAAQYKFFIVACDPPQKSDPPYKLVPVHLDSIL Predict reactive species |
| Full Name | ribonuclease, RNase A family, k6 |
| Calculated Molecular Weight | 150 aa, 17 kDa |
| GenBank Accession Number | BC020848 |
| Gene Symbol | RNASE6 |
| Gene ID (NCBI) | 6039 |
| RRID | AB_3669243 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q93091 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Rnase6, a protein belonging to the superfamily, is considered the only vertebrate specific enzyme known to have a wide range of physiological functions, including digestion, cytotoxicity, angiogenesis, male reproduction, and host defense. Rnase6 expression was upregulated in the peripheral blood and plaque tissues of AS patients (PMID: 34395402).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNASE6 antibody 16425-1-AP | Download protocol |
| IHC protocol for RNASE6 antibody 16425-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RNASE6 Polyclonal antibody (Cat# 16425-1-AP) has 1 citation. It appears in Cell Reports (2026), in a study on synergistic degradation of circular RNAs by RNAseK and lysosomes, applied via immunofluorescence in human samples. The research field encompasses RNA biology and lysosomal degradation pathways.
| Species | Application | Title |
|---|---|---|
