RNASEH2B Polyclonal antibody

RNASEH2B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17527-1-AP

Synonyms

AGS2, DLEU8, Ribonuclease H2 subunit B, ribonuclease H2, subunit B, Ribonuclease HI subunit B, RNase H2 subunit B, RNASEH2B

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Validation Data Gallery

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  WB analysis of mouse thymus using 17527-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 17527-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17527-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17527-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse thymus tissue, HeLa cells, MCF-7 cells, mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17527-1-AP targets RNASEH2B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNASEH2B fusion protein Ag11189
• Full Name: ribonuclease H2, subunit B
• Calculated Molecular Weight: 331 aa, 37 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC036744
• Gene Symbol: RNASEH2B
• Gene ID (NCBI): 79621
• RRID: AB_10597401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides misincorporated during genomic replication. RNASEH2B and RNASEH2C are the non-catalytic subunits of RNase H2 complex. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2), a genetically heterogeneous disease with clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process.

Publications

Species	Application	Title
rat	WB	Nutr Metab (Lond); Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model.; Authors - Shuo Cong