RNASEH2C Polyclonal antibody

RNASEH2C Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16518-1-AP

Synonyms

AGS3, AYP1, Ribonuclease H2 subunit C, ribonuclease H2, subunit C, Ribonuclease HI subunit C, RNase H1 small subunit, RNase H2 subunit C, RNASEH2C

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Validation Data Gallery

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  WB analysis using 16518-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16518-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 16518-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16518-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16518-1-AP

  IP result of anti-RNASEH2C (IP:16518-1-AP, 3ug; Detection:16518-1-AP 1:500) with HEK-293 cells lysate 3200ug.

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  IHC staining of human brain using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, human spleen tissue, human testis tissue, HeLa cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human brain tissue, human lung tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

16518-1-AP targets RNASEH2C in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNASEH2C fusion protein Ag9739
• Full Name: ribonuclease H2, subunit C
• Calculated Molecular Weight: 164 aa, 18 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC023588
• Gene Symbol: RNASEH2C
• Gene ID (NCBI): 84153
• RRID: AB_2181648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions.; Authors - Michal Zimmermann
human	WB	Hum Mol Genet; Reduction of hRNase H2 activity in Aicardi-Goutières Syndrome cells leads to replication stress and genome instability.; Authors - Sara Pizzi
human	WB	J Biol Chem; Analysis of ribonucleotide removal from DNA by human nucleotide excision repair.; Authors - Laura A Lindsey-Boltz