RNASEH2C Polyclonal antibody

RNASEH2C Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16518-1-AP

Synonyms

AGS3, AYP1, Ribonuclease H2 subunit C, ribonuclease H2, subunit C, Ribonuclease HI subunit C, RNase H1 small subunit, RNase H2 subunit C, RNASEH2C



Tested Applications

Positive WB detected inHeLa cells, human testis tissue, human spleen tissue, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue, human lung tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

16518-1-AP targets RNASEH2C in WB, IP, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASEH2C fusion protein Ag9739
Full Name ribonuclease H2, subunit C
Calculated molecular weight 164 aa, 18 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC023588
Gene symbol RNASEH2C
Gene ID (NCBI) 84153
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNASEH2C antibody 16518-1-APDownload protocol
IHC protocol for RNASEH2C antibody 16518-1-APDownload protocol
IP protocol for RNASEH2C antibody 16518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Analysis of ribonucleotide removal from DNA by human nucleotide excision repair.

Authors - Laura A Lindsey-Boltz
humanWB

Hum Mol Genet

Reduction of hRNase H2 activity in Aicardi-Goutières Syndrome cells leads to replication stress and genome instability.

Authors - Sara Pizzi
humanWB

Nature

CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions.

Authors - Michal Zimmermann