RNF135 Polyclonal antibody

RNF135 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25061-1-AP

Synonyms

RIG-I E3 ubiquitin ligase, RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27

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Validation Data Gallery

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  WB analysis of mouse kidney using 25061-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25061-1-AP (RNF135 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 25061-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25061-1-AP (RNF135 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25061-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 25061-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 25061-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 25061-1-AP (RNF135 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 25061-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 25061-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of PC-12 using 25061-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using RNF135 antibody (25061-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, U-87 MG cells
• Positive IHC detected in: human kidney tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

25061-1-AP targets RNF135 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF135 fusion protein Ag18821
• Full Name: ring finger protein 135
• Calculated Molecular Weight: 432 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC005084
• Gene Symbol: RNF135
• Gene ID (NCBI): 84282
• RRID: AB_2879879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ring finger protein 135 (RNF135), located on chromosome 17q11.2, is a RING finger domain-containing E3 ubiquitin ligase that was identified as a bio-marker and therapy target of glioblastoma (PMID: 26856755). RNF135 facilitates RIG-I C-terminal ubiquitination to up-regulate RIG-I-mediated IFN signaling and suppress viral replication. This antibody can detect a band at 48 kDa as predicted.

Publications

Species	Application	Title
human	WB	J Biol Chem; A RIG-I-like receptor directs antiviral responses to a bunyavirus and is antagonized by virus-induced blockade of TRIM25-mediated ubiquitination.; Authors - Yuan-Qin Min
human	WB	Oncol Lett; Ubiquitination of p21 by E3 ligase RNF135 promotes the proliferation of human glioblastoma cells; Authors - Weiting Gu
human	WB,IHC	Cancer Rep (Hoboken); RNF135 Promotes Human Osteosarcoma Cell Growth and Inhibits Apoptosis by Upregulating the PI3K/AKT Pathway; Authors - Bingyao Chen
human	WB,IHC	Environ Toxicol; Epigenetic Regulation of RNF135 by LSD1 Promotes Stemness Maintenance and Brain Metastasis in Lung Adenocarcinoma; Authors - Xiaohan Qu