Tested Applications
| Positive WB detected in | mouse kidney tissue, U-251 cells, U-87 MG cells |
| Positive IHC detected in | human kidney tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-12 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25061-1-AP targets RNF135 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18821 Product name: Recombinant human RNF135 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-210 aa of BC005084 Sequence: MAGLGLGSAVPVWLAEDDLGCIICQGLLDWPATLPCGHSFCRHCLEALWGARDARRWACPTCRQGAAQQPHLRKNTLLQDLADKYRRAAREIQAGSDPAHCPCPGSSSLSSAAARPRRRPELQRVAVEKSITEVAQELTELVEHLVDIVRSLQNQRPLSESGPDNELSILGKENSWKPRLPPHAHCLTRATLHSGELLGLLSGPSIQPLT Predict reactive species |
| Full Name | ring finger protein 135 |
| Calculated Molecular Weight | 432 aa, 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC005084 |
| Gene Symbol | RNF135 |
| Gene ID (NCBI) | 84282 |
| RRID | AB_2879879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IUD6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ring finger protein 135 (RNF135), located on chromosome 17q11.2, is a RING finger domain-containing E3 ubiquitin ligase that was identified as a bio-marker and therapy target of glioblastoma (PMID: 26856755). RNF135 facilitates RIG-I C-terminal ubiquitination to up-regulate RIG-I-mediated IFN signaling and suppress viral replication. This antibody can detect a band at 48 kDa as predicted.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNF135 antibody 25061-1-AP | Download protocol |
| IHC protocol for RNF135 antibody 25061-1-AP | Download protocol |
| WB protocol for RNF135 antibody 25061-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RNF135 Antibody (Cat# 25061-1-AP) has 4 total citations. These appear in Environmental Toxicology, Journal of Biological Chemistry, Cancer Reports, and Oncology Letters. Research fields span epigenetic regulation in lung adenocarcinoma brain metastasis, antiviral innate immune responses, osteosarcoma cell growth via PI3K/AKT signaling, and glioblastoma proliferation through p21 ubiquitination.
| Species | Application | Title |
|---|---|---|
Environ Toxicol Epigenetic Regulation of RNF135 by LSD1 Promotes Stemness Maintenance and Brain Metastasis in Lung Adenocarcinoma | ||
J Biol Chem A RIG-I-like receptor directs antiviral responses to a bunyavirus and is antagonized by virus-induced blockade of TRIM25-mediated ubiquitination. | ||
Cancer Rep (Hoboken) RNF135 Promotes Human Osteosarcoma Cell Growth and Inhibits Apoptosis by Upregulating the PI3K/AKT Pathway | ||
Oncol Lett Ubiquitination of p21 by E3 ligase RNF135 promotes the proliferation of human glioblastoma cells |
