RNF219 Polyclonal antibody

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• RNF219 Polyclonal antibody (24988-1-AP)

RNF219 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 24988-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

C13orf7, EC:2.3.2.27, OBI1, ORC ubiquitin ligase 1, RING finger protein 219

• 

  WB analysis of HEK-293T using 24988-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 24988-1-AP (RNF219 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of A431 using 24988-1-AP

  IP result of anti-RNF219 (IP:24988-1-AP, 4ug; Detection:24988-1-AP 1:300) with A431 cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 24988-1-AP

  IP result of anti-RNF219 (IP:24988-1-AP, 4ug; Detection:24988-1-AP 1:300) with HEK-293 cells lysate 920ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 24988-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24988-1-AP (RNF219 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293T cells
• Positive IP detected in: A431 cells, HEK-293 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24988-1-AP targets RNF219 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21767

  Product name: Recombinant human RNF219 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 271-416 aa of BC136764

  Sequence: CQTALSADGKGSKGSEEDVVSKNQGDSARKQPGSSTSSSSHLAKPSSSRLCDTSSARQESTSKADLNCSKNKDLYQEQVEVMLDVTDTSMDTYLEREWGNKPSDCVPYKDEELYDLPAPCTPLSLSCLQLSTPENRESSVVQAGGS

• Full Name: ring finger protein 219
• Calculated Molecular Weight: 726 aa, 81 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC136764
• Gene Symbol: RNF219
• Gene ID (NCBI): 79596
• RRID: AB_2879832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5W0B1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RING finger protein 219 (RNF219), also known as C13orf7 or OBI1, is an E3 ubiquitin ligase that plays a crucial role in regulating various cellular processes, including inflammation, cell proliferation, DNA replication, and cancer progression. RNF219 is essential for DNA replication origin activation during the S phase.

Publications

What published studies show

The RNF219 Polyclonal antibody (Cat# 24988-1-AP) has 2 total citations. These citations appear in Genomics Proteomics Bioinformatics and J Cell Mol Med. The associated research fields include oral squamous cell carcinoma genomics (repeat expansions and structural variants) and renal tubular epithelial cell autophagy induced by calcium oxalate crystals. Both studies utilized the antibody for Western blot in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Genomics Proteomics Bioinformatics; Long-read Sequencing Reveals Repeat Expansions and Large Structural Variants in Oral Squamous Cell Carcinoma.; Authors - Li Hu
human	WB	J Cell Mol Med; Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals; Authors - Weiming Ma