RNF220 Polyclonal antibody

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  WB analysis using 32482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32482-1-AP (RNF220 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

RNF220 Polyclonal Antibody for WB, ELISA

Cat No. 32482-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RING-type E3 ubiquitin transferase RNF220, RING finger protein 220, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF220, C1orf164

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Tested Applications

• Positive WB detected in: RT-4 cells, U-251 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32482-1-AP targets RNF220 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36190

  Product name: Recombinant human RNF220 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 253-566 aa of BC034221

  Sequence: SKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQREGSCMAEDDAVDIEHENNNRFEEYEWCGQKRIRATTLLEGGFRGSGFIMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL

• Full Name: ring finger protein 220
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC034221
• Gene Symbol: RNF220
• Gene ID (NCBI): 55182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5VTB9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF220 is an E3 ubiquitin ligase that plays a crucial role in the ubiquitination process, which targets proteins for degradation by the proteasome. Mutations in RNF220 have been linked to laminopathies, which are characterized by leukodystrophy, ataxia, and deafness. Additionally, RNF220 is involved in the progression of certain cancers, such as acute myeloid leukemia (AML) and colorectal cancer. Studies have shown that RNF220 promotes the proliferation of leukemia cells and reduces the degradation of Cyclin D1 protein through USP22. It also enhances canonical Wnt signaling through USP7-mediated deubiquitination of beta-catenin. (PMID: 32896826)