RNF31 / HOIP Polyclonal antibody, PBS Only

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• RNF31 / HOIP Polyclonal antibody, PBS Only (30797-1-PBS)

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  WB analysis using 30797-1-AP (same clone as 30797-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30797-1-AP (RNF31 / HOIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 30797-1-AP (same clone as 30797-1-PBS)

  WB result of RNF31 / HOIP antibody (30797-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF31 / HOIP transfected HEK-293 cells. This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 30797-1-AP (same clone as 30797-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 / HOIP antibody (30797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RNF31 / HOIP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 30797-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

30797-1-PBS targets RNF31 / HOIP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34505

  Product name: Recombinant human RNF31 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 459-603 aa of BC017376

  Sequence: RDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK

• Full Name: ring finger protein 31
• Calculated Molecular Weight: 1072 aa, 120 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC017376
• Gene Symbol: RNF31
• Gene ID (NCBI): 55072
• RRID: AB_3086418
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96EP0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ring finger protein 31 (RNF31), also named HOIL-1-interacting protein (HOIP), ZIBRA, is an atypical E3 ubiquitin ligase, belongs to ring finger protein (RNF) family. It was first cloned from breast cancer cell line MCF7 and was identified as a classical component in linear ubiquitin assembly complex (LUBAC) to facilitate NFκB signaling transduction. It also plays important regulatory functions in controlling important oncogenic pathways in breast cancer, such as ERα and p53. RNF31 is highly expressed in muscle, heart, and testis. In cells, RNF31 mainly localizes to the cytoplasm. (PMID:27460922)