Filter: AllWBIF/ICC

WB analysis using 86740-1-RR (same clone as 86740-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86740-1-RR (RNF43 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86740-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 86740-1-RR (same clone as 86740-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RNF43 antibody (86740-1-RR, Clone: 251708C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86740-1-PBS in a different storage buffer formulation.

RNF43 Recombinant monoclonal antibody, PBS Only

Name: RNF43 Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 86740-1-PBS

RNF43 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86740-1-PBS

Clone No.251708C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase RNF43, EC:2.3.2.27, ring finger protein 43, RING-type E3 ubiquitin transferase RNF43, RNF124

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

86740-1-PBS targets RNF43 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg6490 Product name: Recombinant Human RNF43 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-197 aa of NM_001305544.1 Sequence: GFGRTGLVLAAAVESERSAEQKAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPRRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWPVVLIWGNDAEKLMEFVYKNQKAHVRIELKEPPAWPDY Predict reactive species
Full Name	ring finger protein 43
Calculated Molecular Weight	86 kDa
Observed Molecular Weight	86 kDa
GenBank Accession Number	NM_001305544.1
Gene Symbol	RNF43
Gene ID (NCBI)	54894
RRID	AB_3745094
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q68DV7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Able™