RNF43 Recombinant monoclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• RNF43 Recombinant monoclonal antibody, PBS Only (86740-1-PBS)

RNF43 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86740-1-PBS

Clone No.251708C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase RNF43, EC:2.3.2.27, ring finger protein 43, RING-type E3 ubiquitin transferase RNF43, RNF124

• 

  WB analysis using 86740-1-RR (same clone as 86740-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86740-1-RR (RNF43 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86740-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 86740-1-RR (same clone as 86740-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86740-1-RR (RNF43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86740-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 86740-1-RR (same clone as 86740-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RNF43 antibody (86740-1-RR, Clone: 251708C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86740-1-PBS in a different storage buffer formulation.

---

Product Information

86740-1-PBS targets RNF43 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6490

  Product name: Recombinant Human RNF43 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 24-197 aa of NM_001305544.1

  Sequence: GFGRTGLVLAAAVESERSAEQKAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPRRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWPVVLIWGNDAEKLMEFVYKNQKAHVRIELKEPPAWPDY

• Full Name: ring finger protein 43
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: NM_001305544.1
• Gene Symbol: RNF43
• Gene ID (NCBI): 54894
• RRID: AB_3745094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q68DV7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RNF43 (ring finger protein 43) is a member of proteins of Goliath and Godzilla families, which bears the characteristic sequence of an ubiquitin ligase and of a trans-membrane region, encoding E3 ubiquitination kinase and with the activity of E3 ubiquitin protein ligase. RNF43 has attracted much attention in the study of Wnt/β-catenin signaling pathway because of its high structural homology with ZNRF3(zinc and ring finger protein 3). RNF43 inhibits Wnt/β-catenin signalling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.