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RNH1 Polyclonal antibody

RNH1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10345-1-AP

Synonyms

Placental RNase inhibitor, PRI, RAI, Ribonuclease inhibitor, RNH, RNH1



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, human brain tissue, human heart tissue, human liver tissue, L02 cells, HepG2 cells, Jurkat cells, human placenta tissue
Positive IP detected inmouse liver tissue, HEK-293T cells, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10345-1-AP targets RNH1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNH1 fusion protein Ag0350
Full Name ribonuclease/angiogenin inhibitor 1
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC000677
Gene Symbol RNH1
Gene ID (NCBI) 6050
RRIDAB_2269733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonuclease/ angiogenin inhibitor (RNH, synonyms: RAI, MGC4569, MGC18200, MGC54054) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization.

Protocols

Product Specific Protocols
WB protocol for RNH1 antibody 10345-1-APDownload protocol
IHC protocol for RNH1 antibody 10345-1-APDownload protocol
IF protocol for RNH1 antibody 10345-1-APDownload protocol
IP protocol for RNH1 antibody 10345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO J

Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.

Authors - Rongpan Bai
humanWB

J Cell Biol

Angiogenin cleaves tRNA and promotes stress-induced translational repression.

Authors - Yamasaki Satoshi S
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers.

Authors - Shozo Honda
humanWB

Int J Mol Sci

RTCB Complex Regulates Stress-Induced tRNA Cleavage

Authors - Yasutoshi Akiyama
humanIHC

J Proteome Res

Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE.

Authors - Stephens Andrew N AN
mouseWB

J Biol Chem

Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress.

Authors - Saikia Mridusmita M