RNH1 Monoclonal antibody

• Featured Product
• KD/KO Validated

RNH1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

4A1C4

Cat no : 66028-1-Ig

Synonyms

Placental RNase inhibitor, PRI, RAI, Ribonuclease inhibitor, RNH, RNH1

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Validation Data Gallery

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  WB analysis of HeLa using 66028-1-Ig

  HeLa, Jurkat, L02 cells were subjected to SDS PAGE followed by western blot with 66028-1-Ig (RNH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 66028-1-Ig

  WB result of RNH1 antibody (66028-1-Ig, 1:4000) with si-control and si-RNH1 transfected HeLa cells.

• 

  WB analysis of L02 using 66028-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66028-1-Ig (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66028-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66028-1-Ig (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66028-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66028-1-Ig (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66028-1-Ig is RNH1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RNH1 fusion protein Ag18093
• Full Name: ribonuclease/angiogenin inhibitor 1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC000677
• Gene Symbol: RNH1
• Gene ID (NCBI): 6050
• RRID: AB_11043338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonuclease/ angiogenin inhibitor (RNH, synonyms: RAI, MGC4569, MGC18200, MGC54054) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization.