ROBO2 Polyclonal antibody

ROBO2 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19986-1-AP

Synonyms

KIAA1568, ROBO2, Roundabout homolog 2, SAX3

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Validation Data Gallery

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  IP experiment of SH-SY5Y using 19986-1-AP

  IP result of anti-ROBO2 (IP:19986-1-AP, 4ug; Detection:19986-1-AP 1:300) with SH-SY5Y cells lysate 3600ug.

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  IHC staining of human cerebellum using 19986-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19986-1-AP (ROBO2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 19986-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19986-1-AP (ROBO2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 19986-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19986-1-AP (ROBO2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 19986-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19986-1-AP (ROBO2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human cerebellum tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19986-1-AP targets ROBO2 in IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: roundabout, axon guidance receptor, homolog 2 (Drosophila)
• Calculated Molecular Weight: 151 kDa
• GenBank Accession Number: NM_002942
• Gene Symbol: ROBO2
• Gene ID (NCBI): 6092
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROBO2, also named as KIAA1568, belongs to the immunoglobulin superfamily and ROBO family. ROBO2 is a receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Defects in ROBO2 are the cause of vesicoureteral reflux type 2 (VUR2). A chromosomal aberration involving ROBO2 is a cause of multiple congenital abnormalities, including severe bilateral VUR with ureterovesical junction defects. The antibody recognizes the C-term of ROBO.