ROBO4-Specific Polyclonal antibody

ROBO4-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20221-1-AP

Synonyms

ECSM4, Magic roundabout, MRB, ROBO4, ROBO4-Specific, Roundabout homolog 4

---

Validation Data Gallery

• 

  WB analysis of HeLa using 20221-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20221-1-AP (ROBO4-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20221-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20221-1-AP (ROBO4-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

20221-1-AP targets ROBO4-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: roundabout homolog 4, magic roundabout (Drosophila)
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 90-170 kDa
• GenBank Accession Number: NM_019055
• Gene Symbol: ROBO4
• Gene ID (NCBI): 54538
• RRID: AB_10665944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROBO4 belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins. This antibody is specific to ROBO4.

Publications

Species	Application	Title
mouse	WB	Cell Mol Biol Lett; Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions; Authors - Seyram Yao Adzraku
human,mouse	WB	J Transl Med; The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy; Authors - Liangliang Zhao
human	WB	Mol Ther Nucleic Acids; Role of ANKHD1/LINC00346/ZNF655 Feedback Loop in Regulating the Glioma Angiogenesis via Staufen1-Mediated mRNA Decay.; Authors - Chunqing Yang