ROCK2(middle) Polyclonal antibody

• Featured Product
• KD/KO Validated

ROCK2(middle) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 21645-1-AP

Synonyms

ROCK2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2

---

Validation Data Gallery

• 

  WB analysis of HT-1080 using 21645-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21645-1-AP

  WB result of ROCK2(middle) antibody (21645-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2(middle) transfected HeLa cells.

• 

  WB analysis of HeLa using 21645-1-AP

  WB result of ROCK2(middle) antibody (21645-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2(middle) transfected HeLa cells.

• 

  WB analysis of mouse brain using 21645-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21645-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21645-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21645-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21645-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21645-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21645-1-AP

  IP result of anti-ROCK2(middle) (IP:21645-1-AP, 5ug; Detection:21645-1-AP 1:700) with HeLa cells lysate 1320ug.

• 

  IHC staining of human liver using 21645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 21645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 21645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 21645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 21645-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 21645-1-AP

  Immunofluorescent analysis of Hela cells, using ROCK2 antibody 21645-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HT-1080 cells, mouse brain tissue, HeLa cells, mouse lung tissue, MCF-7 cells, mouse liver tissue, L02 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 21 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Product Information

21645-1-AP targets ROCK2(middle) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ROCK2(middle) fusion protein Ag16330
• Full Name: Rho-associated, coiled-coil containing protein kinase 2
• Calculated Molecular Weight: 77 kDa, 161 kDa
• Observed Molecular Weight: 161 kDa
• GenBank Accession Number: BC111801
• Gene Symbol: ROCK2
• Gene ID (NCBI): 9475
• RRID: AB_10858624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).

Publications

Species	Application	Title
human	IHC	Oncotarget; Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.; Authors - Rachel Hiu Ha Ching
rat	WB,IF	Neurochem Res; Pre-electroacupuncture Ameliorates Cerebral Ischemia-reperfusion Injury by Inhibiting Microglial RhoA/pyrin/GSDMD Signaling Pathway; Authors - Hao Fang
human	WB	Cells; The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.; Authors - Kathryn N Becker
rat	WB	Front Pharmacol; Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus.; Authors - Penghui Yuan
mouse	WB	Mol Pharmacol; Megakaryocytic Smad4 Regulates Platelet Function Through Syk and ROCK2 Expression.; Authors - Yanhua Wang
rat	WB	J Sex Med; FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.; Authors - Kai Cui