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- KD/KO Validated
ROCK2-Specific(C-term) Polyclonal antibody
ROCK2-Specific(C-term) Polyclonal Antibody for IF, IHC, WB,ELISA
Cat no : 20248-1-AP
|Positive WB detected in||HeLa cells, mouse brain tissue, rat brain tissue|
|Positive IHC detected in||human colon tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
20248-1-AP targets ROCK2-Specific(C-term) in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||Rho-associated, coiled-coil containing protein kinase 2|
|Calculated molecular weight||161 kDa|
|Observed molecular weight||164 kDa|
|GenBank accession number||NM_004850|
|Gene ID (NCBI)||9475|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.
|Product Specific Protocols|
|WB protocol for ROCK2-Specific(C-term) antibody 20248-1-AP||Download protocol|
|IHC protocol for ROCK2-Specific(C-term) antibody 20248-1-AP||Download protocol|
|IF protocol for ROCK2-Specific(C-term) antibody 20248-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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