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  • KD/KO Validated

ROCK2-Specific(C-term) Polyclonal antibody

ROCK2-Specific(C-term) Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20248-1-AP

Synonyms

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20248-1-AP targets ROCK2-Specific(C-term) in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Rho-associated, coiled-coil containing protein kinase 2
Calculated molecular weight 161 kDa
Observed molecular weight 164 kDa
GenBank accession numberNM_004850
Gene symbol ROCK2
Gene ID (NCBI) 9475
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.

Protocols

Product Specific Protocols
WB protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
IHC protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
IF protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Acta Biomater

ROCK isoforms differentially modulate cancer cell motility by mechanosensing the substrate stiffness.

Authors - Yueting Peng
  • KD Validated
mouseWB,IHC

Cell Death Dis

Suppressing the Na+/H+ exchanger 1: a new sight to treat depression.

Authors - Xueyang Deng
mouseIF

Neuropharmacology

Modulation of LPA1 receptor-mediated neuronal apoptosis by Saikosaponin-d: A target involved in depression.

Authors - Lixing Xu
humanWB

Biochem Biophys Res Commun

MiR-455-3p suppresses renal fibrosis through repression of ROCK2 expression in diabetic nephropathy.

Authors - Jian Wu
humanWB,IHC

Brain Dev

Activation of leukocyte immunoglobulin-like receptor B2 signaling pathway in cortical lesions of pediatric patients with focal cortical dysplasia type IIb and tuberous sclerosis complex.

Authors - Jiong Yue
ratWB,IHC

Am J Transl Res

Effects of chemerin/CMKLR1 in obesity-induced hypertension and potential mechanism.

Authors - Chunyan Weng