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ROCK2-Specific(C-term) Polyclonal antibody

ROCK2-Specific(C-term) Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20248-1-AP

Synonyms

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20248-1-AP targets ROCK2-Specific(C-term) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Rho-associated, coiled-coil containing protein kinase 2
Calculated Molecular Weight 161 kDa
Observed Molecular Weight 164 kDa
GenBank Accession NumberNM_004850
Gene Symbol ROCK2
Gene ID (NCBI) 9475
RRIDAB_10665551
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.

Protocols

Product Specific Protocols
WB protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
IHC protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
IF protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomaterials

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Authors - Hongyuan Song
humanWB,IHC

Cell Prolif

The distinct roles of myosin IIA and IIB under compression stress in nucleus pulposus cells.

Authors - Wencan Ke
humanWB,IHC,IF

Exp Neurol

PMID: 27637803

Authors - Jiong Yue
humanWB

Chin J Nat Med

Stigmasterol protects human brain microvessel endothelial cells against ischemia-reperfusion injury through suppressing EPHA2 phosphorylation

Authors - Suping Li
ratIHC

Oxid Med Cell Longev

Lycopene Ameliorates Transplant Arteriosclerosis in Vascular Allograft Transplantation by Regulating the NO/cGMP Pathways and Rho-Associated Kinases Expression.

Authors - Yunqiang He
mouseWB

ASN Neuro

Induction of oligodendrocyte differentiation and in vitro myelination by inhibition of rho-associated kinase.

Authors - Carlos E Pedraza
  • KD Validated