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  • KD/KO Validated

ROCK2-Specific(C-term) Polyclonal antibody

ROCK2-Specific(C-term) Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 20248-1-AP


KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20248-1-AP targets ROCK2-Specific(C-term) in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Rho-associated, coiled-coil containing protein kinase 2
Calculated molecular weight 161 kDa
Observed molecular weight 164 kDa
GenBank accession numberNM_004850
Gene symbol ROCK2
Gene ID (NCBI) 9475
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.


Product Specific Protocols
WB protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
IHC protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
IF protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Acta Biomater

ROCK isoforms differentially modulate cancer cell motility by mechanosensing the substrate stiffness.

Authors - Yueting Peng
  • KD Validated

Cell Death Dis

Suppressing the Na+/H+ exchanger 1: a new sight to treat depression.

Authors - Xueyang Deng


Modulation of LPA1 receptor-mediated neuronal apoptosis by Saikosaponin-d: A target involved in depression.

Authors - Lixing Xu

Biochem Biophys Res Commun

MiR-455-3p suppresses renal fibrosis through repression of ROCK2 expression in diabetic nephropathy.

Authors - Jian Wu

Brain Dev

Activation of leukocyte immunoglobulin-like receptor B2 signaling pathway in cortical lesions of pediatric patients with focal cortical dysplasia type IIb and tuberous sclerosis complex.

Authors - Jiong Yue

Am J Transl Res

Effects of chemerin/CMKLR1 in obesity-induced hypertension and potential mechanism.

Authors - Chunyan Weng