Tested Applications
| Positive WB detected in | human brain tissue, human kidney tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17047-1-AP targets ROGDI in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10688 Product name: Recombinant human ROGDI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-287 aa of BC012901 Sequence: MATVMAATAAERAVLEEEFRWLLHDEVHAVLKQLQDILKEASLRFTLPGSGTEGPAKQENFILGSCGTDQVKGVLTLQGDALSQADVNLKMPRNNQLLHFAFREDKQWKLQQIQDARNHVSQAIYLLTSRDQSYQFKTGAEVLKLMDAVMLQLTRARNRLTTPATLTLPEIAASGLTRMFAPALPSDLLVNVYINLNKLCLTVYQLHALQPNSTKNFRPAGGAVLHSPGAMFEWGSQRLEVSHVHKVECVIPWLNDALVYFTVSLQLCQQLKDKISVFSSYWSYRPF Predict reactive species |
| Full Name | rogdi homolog (Drosophila) |
| Calculated Molecular Weight | 287 aa, 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC012901 |
| Gene Symbol | ROGDI |
| Gene ID (NCBI) | 79641 |
| RRID | AB_2182328 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZN7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ROGDI encoded by the ROGDI gene, which encodes a leucine-zipper protein with high expression in the human spinal cord and brain, acts as a positive regulator of cell proliferation. Defections of ROGDI are the cause of Kohlschuetter-Toenz syndrome (KTZS)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ROGDI antibody 17047-1-AP | Download protocol |
| IP protocol for ROGDI antibody 17047-1-AP | Download protocol |
| WB protocol for ROGDI antibody 17047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ROGDI Polyclonal antibody (catalog number 17047-1-AP) has 7 total citations. These appear in Nature Structural & Molecular Biology, Cell Reports, American Journal of Human Genetics, Cancer Biology & Therapy, and Scientific Reports. The associated research fields include V-ATPase protein complex assembly, cross-vertebrate brain protein interaction mapping, Kohlschütter-Tönz syndrome (a genetic disorder linked to ROGDI mutations), cervical cancer radiosensitivity, and presynaptic protein function. Applications span Western blot, immunoprecipitation, immunofluorescence, and immunohistochemistry across human, mouse, pig, rat, and Drosophila species.
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol A heterotrimeric protein complex assembles the metazoan V-ATPase upon dissipation of proton gradients | ||
Cell Rep A cross-vertebrate brain protein interaction map identifies conserved neural and non-neural complexes. | ||
Am J Hum Genet A nonsense mutation in the human homolog of Drosophila rogdi causes Kohlschutter-Tonz syndrome. | ||
Cancer Biol Ther Downregulation of a novel human gene, ROGDI, increases radiosensitivity in cervical cancer cells.
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