RORA Polyclonal antibody

• Featured Product
• KD/KO Validated

RORA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 10616-1-AP

Synonyms

NR1F1, Nuclear receptor ROR alpha, Nuclear receptor RZR alpha, RAR related orphan receptor A, RORA, RZR ALPHA, RZRA

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Validation Data Gallery

• 

  WB analysis using 10616-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10616-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10616-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10616-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10616-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, PC-3 cells, K-562 cells, Jurkat cells, L02 cells, SMMC-7721 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

10616-1-AP targets RORA in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse , rat samples.

• Tested Reactivity: human, mouse , rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RORA fusion protein Ag0944
• Full Name: RAR-related orphan receptor A
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC008831
• Gene Symbol: RORA
• Gene ID (NCBI): 6095
• RRID: AB_2253844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].

Publications

Species	Application	Title
mouse	WB	Nat Commun; A proteomics landscape of circadian clock in mouse liver.; Authors - Yunzhi Wang
mouse	WB	Free Radic Biol Med; Hydrogen peroxide modulates clock gene expression via PRX2-STAT3-REV-ERBα/β pathway.; Authors - Guohua Ji ; KD Validated
mouse	WB	J Cell Physiol; MCPIP1 regulates RORα expression to protect against liver injury induced by lipopolysaccharide via modulation of miR-155.; Authors - Zhenzhen Li
human	WB	Int Immunopharmacol; ILC2s induce adaptive Th2-type immunity in different stages of tuberculosis through the Notch-GATA3 pathway.; Authors - Qifeng Li
rat	WB	Front Physiol; PMID: 26696906; Authors - You-Lin Tain
rat	WB	Biol Reprod; Maternal melatonin or agomelatine therapy prevents programmed hypertension in male offspring of mother exposed to continuous light.; Authors - You-Lin Tain